First, the principle of ozone disinfection:

Ozone is recognized as the second strongest oxidant after fluorine. At a certain concentration, it can trigger biochemical oxidation reactions with microorganisms such as bacteria and viruses. Ozone can interfere with the glucose oxidase that bacteria need to break down glucose, thereby disrupting their metabolic processes. It directly interacts with microorganisms, damaging their organelles and ribonucleic acids (RNA), and breaking down large molecules like DNA, RNA, proteins, lipids, and polysaccharides. This interference disrupts bacterial growth and function. Additionally, ozone can penetrate the cell membrane, interact with the outer membrane lipoproteins and inner lipopolysaccharides, leading to membrane permeabilization and ultimately causing the cell to rupture and die. It also inactivates genetic materials, viruses, phages, mycoplasma, and pyrogens (endotoxins) within the dead cells. The effect of ozone on microorganisms can be categorized into three types: bacteriostatic, bactericidal, and lytic. Ozone sterilization primarily functions as a bacteriostatic agent.

Due to its strong disinfecting and sterilizing properties, ozone is widely used in medical treatments across various diseases in Western countries. Several factors influence the effectiveness of ozone sterilization, including concentration, temperature, and humidity, though the duration of exposure has less impact. Once the ozone concentration reaches a critical threshold, disinfection can occur almost instantly. When used for fumigation, higher humidity levels enhance the sterilization effect. Studies have shown that when relative humidity is below 35%, even high ozone concentrations may not effectively kill airborne bacteria. As temperature increases, the bactericidal efficiency of ozone improves, but this also reduces its solubility in water. Therefore, maintaining a temperature around 40 ± 5°C is ideal for optimal sterilization results.

Second, the role of ozone in killing microorganisms:

1. Bacterial propagules: Ozone demonstrates high efficacy against various bacteria. For instance, it can achieve a killing rate of over 99.99% against Pseudomonas aeruginosa, and 100% against Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, F. sinensis, and Vibrio cholerae.

2. Bacterial spores: Under specific conditions, ozone can achieve a 99.99% killing rate against Bacillus subtilis spores.

3. Viruses: Ozone can effectively inactivate a wide range of viruses. For example, after 30 minutes of exposure, ozone can reduce the titer of hepatitis B surface antigen by half, completely destroy hepatitis A virus antigens, and inactivate 99% of the influenza A virus in the air at a concentration of 0.5 ppm. Poliovirus type I can be fully inactivated at an ozone concentration of 0.13 mg/L. According to foreign studies, at a concentration of 4 mg/L, ozone can completely inactivate HIV at a titer of 106 CID50/ml, reducing the viral titer by six logarithmic units.

4. Fungi: Ozone is highly effective in inactivating various molds. It achieves a 100% kill rate against fungi such as Mucor, black mold, and white mold.

The "Kangkang Shen" gynecological treatment device was tested in 2005 at the Second Affiliated Hospital of Sun Yat-sen University, one of the national clinical trial bases. It underwent rigorous pathology, virus, and efficacy testing, along with random sampling from 30 patients. These included 20 cases of vaginitis (classified into trichomonas, bacterial, and other types) and 10 cases of cervicitis (divided into degrees of cervical erosion). Compared to conventional treatment methods, the device showed significant improvement in symptoms and overall effectiveness. It was particularly effective in treating various types of vaginitis, with high patient satisfaction. The device is safe, compact, lightweight, non-toxic, and free of side effects, making it convenient for use anytime and anywhere. It represents a new approach in the treatment of gynecological conditions.

Applicable Diseases:

It is suitable for the treatment of women suffering from streptococcal (mold) vaginitis, trichomonas vaginitis, bacterial vaginitis, and chronic cervical inflammation. Clinical Results: After several trials, the total effective rate for vaginitis reached 90% (P > 0.05), which is comparable to drug treatment. For cervicitis, the total effective rate was 83.33%.

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