First, the principle of ozone disinfection:

Ozone is recognized as the second strongest oxidizing agent after fluorine. At a certain concentration, it can trigger biochemical oxidation reactions with microorganisms such as bacteria and viruses. Ozone can oxidize and break down essential enzymes like glucose oxidase in bacteria, which are crucial for their metabolism. It directly interacts with microorganisms, damaging their organelles and ribonucleic acids (RNA), and degrading large molecules such as DNA, RNA, proteins, lipids, and polysaccharides. This disrupts bacterial metabolism and growth. Additionally, ozone can penetrate cell membranes, invade the outer membrane, and interact with lipoproteins and lipopolysaccharides, causing the cell to become permeable and eventually lyse. This process also leads to the denaturation and death of genetic material, parasites, viruses, phages, mycoplasma, and pyrogens (endotoxins). The effect of ozone on microorganisms can be categorized into three types: bacteriostatic, bactericidal, and lytic. Ozone sterilization primarily functions as a bacteriostatic agent.

Due to its powerful disinfecting and sterilizing properties, ozone is widely used in medical treatments across Western countries. The effectiveness of ozone sterilization depends mainly on factors such as concentration, temperature, and humidity, but less so on contact time. Once the ozone concentration reaches a certain threshold, disinfection can occur almost instantly. When used for fumigation, higher humidity enhances the sterilization effect. Studies have shown that when relative humidity is below 35%, even high ozone concentrations may not effectively kill airborne bacteria. As temperature increases, the bactericidal efficiency of ozone improves, but higher temperatures reduce its solubility in water. Therefore, the optimal temperature range for ozone sterilization is around 40 ± 5°C to ensure effective results.

Second, the role of ozone in killing microorganisms:

1. Against bacterial propagules: Ozone has a killing rate exceeding 99.99% against *Pseudomonas aeruginosa*, and 100% against *Staphylococcus aureus*, *Escherichia coli*, *Salmonella typhimurium*, *F. sinensis*, and *Vibrio cholerae*.

2. Against bacterial spores: Under specific conditions, ozone can achieve a 99.99% killing rate against *Bacillus subtilis* spores.

3. Against viruses: Ozone can inactivate various viruses. For example, after 30 minutes of exposure, ozone can reduce the titer of hepatitis B surface antigen by half. It completely destroys hepatitis A virus antigens at 100% efficiency. With a concentration of 0.5 ppm, ozone can inactivate 99% of the influenza virus in the air. Poliovirus type I can be fully inactivated at 0.13% mg/l. According to foreign data, at a concentration of 4 mg/L, the titer of HIV virus can be reduced by six logarithms.

4. Against fungi: Ozone is highly effective against mold. It achieves a 100% killing rate against *Mucor*, black, and white molds.

The "Kangkang Shen" gynecological treatment device was tested in 2005 by the Second Affiliated Hospital of Sun Yat-sen University, a national clinical trial base. A total of 30 patients were randomly selected, including 20 with vaginitis (divided into trichomonas, bacterial, and other types) and 10 with cervicitis (classified as mild or moderate cervical erosion). Compared to conventional treatment methods, the symptoms significantly improved after the trial period, showing marked effectiveness. Clinically, this treatment was particularly effective for certain types of vaginitis. The device is safe, compact, lightweight, non-toxic, and free of side effects. It is not restricted by time or location, making it a new and promising approach for gynecological treatments.

Applicable Diseases:

This treatment is suitable for women suffering from streptococcal (mold) vaginitis, trichomonas vaginitis, bacterial vaginitis, and chronic cervical inflammation.

Clinical Effect:

After several clinical trials, the overall effective rate for vaginitis reached 90% (P > 0.05), which is comparable to drug treatment. The overall effective rate for cervicitis was 83.33%. This information is sourced from the original article on Kang Kang God: /info/2009723953

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