Electrophoresis tank common problem solution
<Fault 1> The leakage of the platinum electrode hole in the manual bonding electrophoresis tank may be due to the long working time of the electrophoresis tank, and the plugging glue (condensed liquid) should be used for sealing.
<Fault 2> Leakage at the electrode hole of the mold horizontal electrophoresis tank Check whether the seal ring under the electrode is screwed in the correct position or damaged. Replace the seal ring if it is damaged.
<Fault 3> Electrolytic cell organic plate bonding site leakage or open plastic plate opening is not suitable. If it is found that we can open the glue, we can use chloroform for local bonding. After the adhesive is solidified, use the plugging adhesive. (Condensate) can be blocked.
<Fault 4> Electrophoresis tank seal strip leakage (vertical tank)
Under normal circumstances, the narrower sealing strip of the electrophoresis tank is not easy to leak, otherwise the wider one is easier to leak.
1. Whether the sealing strip is aging or damaged. If it has been aged or damaged, the sealing strip should be replaced.
2. If the sealing strip is intact, visually inspect the sealing strip for unevenness. If it is necessary, remove the sealing strip for reinstallation. When installing, be careful not to pull the sealing strip so that it is naturally embedded in the sealing groove, and ensure uniform installation and avoid the unevenness of the sealing strip.
3. If the buffer leaks from the middle of the sealing strip, it means that the leak is more concave than the two sides (not on the same horizontal line). We need to remove the sealing strip, clean the adhesive in the tank, and then thicken the narrow side. A strip (such as 0.4 mm) is glued into the groove to pad the concave sealing strip so that it conforms to both sides, and then the sealing strip is adhered to the groove.
<Fault 5> No current in the electrophoresis tank (no bubbles on the platinum tight electrode)
1. First check the electrophoresis instrument for output voltage with a multimeter (note the DC voltage block, the output must not exceed the range to avoid damage to the multimeter)
2. Check whether the electrophoresis output line is turned on by using a multimeter electric block.
3. Use the multimeter electric block to check whether the electrode socket of the electrophoresis tank and the platinum wire are reliably connected. It is important to check whether the output socket and the plug are tightly connected with the solder piece connected to the platinum wire. If it is loose, use a needle-nosed pliers to fasten it. If it is not current, check whether the platinum wire is broken. If the platinum wire has signs of breakage, solder it with a soldering iron. First, apply the flux on the platinum wire and then solder it with a soldering iron. Two pieces of platinum wire can be welded together.

<Fault 6> The heat dissipation effect of the aluminum plate is not good. Firstly, there is no gap between the aluminum plate and the glass plate. If there is a gap, the bolt should be tightened first. If the aluminum plate and the glass plate cannot be flattened by the strong bolt, the aluminum plate can be removed. Clean the dirt on the surface, and pad the aluminum plate with the corresponding thickness of the gasket according to the size of the gap, and ensure that the four sides of the aluminum plate are flat (the flat aluminum plate should be lower than the sealing strip 3-4 mm).

Five, common problems and solutions
1, texture and tailings: due to poor dissolution of the sample, the method of overcoming can be centrifuged before loading, adding some solubilizing auxiliary reagents such as urea.
2. The protein band is too wide. When it is connected to the protein band of the adjacent lane, the amount of sample loading can be reduced due to too much sample loading.
3. The electrophoresis time is longer than normal. Possibly due to incorrect pH selection of the gel buffer system and the electrode buffer system, that is, the difference between the pH of the buffer system and the isoelectric point of the separated substance is too small, or the ionic strength of the buffer system is too high.
4. The indicator is a smile symbol (the front edge of the indicator shows an upward curve), indicating that the gel is unevenly cooled, and the middle part is not well cooled, resulting in different mobility of the molecules.
5, the indicator into a crying face symbol (the front edge of the indicator shows a downward curve), because the device of the electrophoresis tank is not suitable, especially the gel at the bottom of the "sandwich" composed of gel and glass plate has bubbles or gel close to the septum The polymerization is not complete.
6, the gel time is wrong. Usually the gel is condensed in 60 min, 1H. If it is too slow, it may be TEMEN. The dose of APS is insufficient or invalid. APS should be used now, TEMED is unstable and easily oxidized to yellow. If the condensation is too fast, the amount of APS and TEMED may be too much. At this time, the glue is too hard and easy to crack, and it is easy to burn when electrophoresis.

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