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Neural stem cells (NSCs) have the ability to self-renew, differentiate into multiple cells, and migrate to the site of injury. Tissues from multiple sources can develop into neural stem cells, such as fetal tissue and embryonic stem cells. In the treatment of damaged central nervous system, current research suggests that neural stem cells can be used to replace damaged central nervous system, such as damaged spinal nerve cells. Although neural stem cells can effectively promote the functional recovery process, these NSCs tend to develop very unevenly, showing different characteristics depending on the source.
The emergence of induced pluripotent stem cells (iPS cells) technology has provided new hope for the development of regenerative medicine. The researchers used a viable and stable method to obtain long-term stable, self-renewing neuroepithelial stem cells from human iPS cells (hiPS-lt-NES cells). The obtained neural stem cells have good homogeneity and very similar characteristics and can be used for standardized analysis. In this study, the researchers found that hiPS-lt-NES cells successfully transplanted to damaged mouse spinal cells can differentiate into a variety of neural cell populations and promote the recovery of damaged lower extremity motor function. In further studies, neurons induced by hiPS-lt-NES cells were co-transplanted with neurons that survived in vivo, which can repair damaged functions more effectively. Two types of neuronal cells form new prominent corpuscles and neuronal circuits that regenerate the corticospinal tract.
The results of this study indicate that hiPS-lt-NES cell transplantation is a promising development in the treatment of spinal cord injury as an effective cell therapy.
The MED64 planar microelectrode array recording system is a complete and easy-to-use multi-channel isolated extracellular neuroelectrophysiological research tool produced by Alpha Med Science, Japan. The system includes a complete set of hardware equipment consumables and analysis software required for in vitro electrophysiological studies. The user can conveniently and quickly perform electrophysiological examination on ex vivo materials such as brain slices, myocardial sections, cultured nerve cells or cardiomyocytes, stem cells, etc. The cells or tissues to be cultured directly on the electrode coating material can be allowed to measure. Local electrical changes directly adjacent to the cell, while simultaneously recording the electrophysiological signals of multiple cells.
Application of multi-channel array system (Alpha MED64 system) in restoring damaged lower limb nerve cell therapy
A team of neuroscientists from Japan, the United Kingdom, and Indonesia transplanted neural stem cells induced by iPS cells into the injured spine of mice, using a multi-channel array system (Alpha MED64 system) as the core research platform. It solves the problem of poor homogeneity of common neural stem cell transplantation and effectively promotes the recovery of lower limb function in mouse models. The results of this research were published in the recently published famous publication "STEM CELLS" (Stem Cells 2012; 30:1163–1173 ).
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