Semen quality testing standards and methods

<

One Kind of Rooster Semen Appearance Quality Testing

Ten cockerels were randomly collected from 100 cocks in each house.

1.1 Ejaculation amount: The collected semen was measured with a calibrated harvesting cup. The average number of selected roosters was 0.4-1 ml.

1.2 Semen Color: Normal fresh cock semen is milky white. The higher the semen density, the thicker the milky white, and the lower the semen density, the lighter the color. Pink mixed with blood; yellowish brown mixed with feces; white cotton wool mixed with urate; transparent fluid-like mixed with water; In short, those who have abnormal color should not be used for insemination.

Two kinds of cocks semen microscopy

2.1 Density Estimation Method: Firstly mix the semen. Take a clean slide and add a small drop of semen on one end with a sterile pipette. When dropping the sample, lean the dropper on the slide, then Moving backwards, be careful not to tighten too tightly. Spread the sample evenly on the glass slide. Do not use a pipette to agitate the liquid level on the slide. During the microscopic examination, four mirrors in different directions were selected and observed at a low magnification of 160 times. The following three conditions are generally observed:

"Dense": refers to the microscope, the entire field of vision is completely filled with sperm, almost no gap between the sperm and a single sperm activity. At this time, the density of sperm is more than 4 billion per milliliter.

"Medium" refers to the apparent gap between the spermatozoa in the field of vision that is equivalent to the length of a sperm. The activity of a single sperm can be seen. At this time, the sperm density is 2 to 400 million milliliters per milliliter.

"Diluted" refers to a large gap between spermatozoa within the field of vision, which can accommodate two or more spermatozoa with a semen density of less than 2 billion cells per milliliter. Such semen should not be used as much as possible.

2.2 Sperm vitality

If a large group of sperm under the microscope looks like boiled water or a tumbling motion in the form of a cloud, it can be judged that the vigor of the sperm is better. Therefore, the test is based on the ability to check the movement of the sperm and thus the vigor of the sperm. During the microscopic examination, four mirrors in different directions were selected and observed at a low magnification of 160 times. Generally can be judged as follows:

Better vigor: In the selected four mirrors, at least three mirrors saw the movement of large groups of sperm;

Vitality: There are two fields of vision to observe the movement of large groups of sperm;

Poor viability: Only one field of vision can see how large groups of sperm swim.

If the first test of the cock's semen is poorly vibrated and the test is repeated once again, if it is still unqualified, it can be judged as having poor vigor.

LED9 Operating Theatre Lights

Led Surgical Light,Operating Theatre Lights,Operation Theatre Lights,Led9 Operating Theatre Lights

NINGBO TECHART MEDICAL EQUIPMENT CO.,LTD , https://www.techartmed.com