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Selection of gel imaging analysis system
Key words:
Gel imaging analysis system chemiluminescence imaging analysis system, multicolor fluorescence imaging analysis system, multifunctional living imaging analysis system
I. Introduction:
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With the gradual deepening of biological research and the deepening of biological related interdisciplinary research on molecular biology,
The practical application of molecular imaging analysis systems is gradually becoming more and more popular, but nowadays, it is a difficult problem for the majority of users to face the selection of many imported and domestic molecular imaging imaging analysis systems.
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Second, the molecular imaging imaging analysis system includes an imaging system and an analysis software system,
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The imaging system includes: camera, darkroom, transilluminator, UV and white illumination, amber filter slide and UV shield. The camera can be divided into low to high according to CCD: about 450,000, about 1 million. , about 1.4 million, about 2 million, about 3 million, about 4 million, about 5 million, and even higher, according to the temperature of CCD can be divided into normal temperature CCD camera, excellent refrigeration CCD camera, these two compared Excellent refrigeration CCD camera is superior to the excellent refrigeration CCD camera in terms of sensitivity and resolution as well as external influence on the experiment. The excellent cooling CCD camera is superior to the future development trend and must be required for the excellent molecular imaging imaging analysis system.
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Third, the software part is written by Wu Wensheng, analysis software system, and can be roughly divided into 1D software, 2D software
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Fourth, according to the application range of molecular imaging imaging analysis system, it can be divided into common gel imaging analysis system, chemiluminescence imaging analysis system, multi-color fluorescence imaging analysis system, and multi-function living body imaging analysis system.
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1 Ordinary gel imaging analysis system: protein electrophoresis gel, DNA gel, sample for image acquisition and qualitative and quantitative analysis, samples include: EB, SYBR Green, SYBR Gold, Texas Red, GelStar, Fluoroscecin, Radiant Red Equal-stained nucleic acid monitoring; and Coomassie Blue, SYPRO Orange, various stained protein gels such as test dyes. (or UV, EB and colored and visible sample imaging)
2 Chemiluminescence Imaging Analysis System: Imaging range covers: UV, EB, chemiluminescence, UV-fluorescence, colored and visible sample imaging
3 multi-color fluorescence imaging analysis system: imaging range covers: UV, EB, chemiluminescence, multi-color fluorescence, colored and visible sample imaging
4 multi-function in vivo imaging analysis system: UV, EB, chemiluminescence, multi-color fluorescent fluorescence, colored and visible sample imaging and isolated tissues and small animals, and large animals
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Five, choose
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The choice of molecular imaging imaging analysis systems now has such a trend:
1 Specialized research Molecular biology has been equipped with a common gel imaging analysis system. In the past two years, some laboratories with visionary research or research into the direction of chemiluminescence imaging and multicolor fluorescence imaging have been assembled. Chemiluminescence imaging analysis System, multi-color fluorescence imaging analysis system, this part has already been a certain number. Very few laboratories are equipped with a multi-function in vivo imaging analysis system, which is a very small number in the country.
2 The second category is the research and biology-related crossover and marginal discipline laboratories. It has just assembled a common gel imaging analysis system, and this part of the teacher has not yet fully understood and systematically operated the molecular imaging imaging analysis system. The imaging imaging analysis system is even less of an idea.
3 From the current development direction, professional research in molecular biology has begun to trend toward chemiluminescence imaging analysis systems based on common gel imaging analysis systems, and multicolor fluorescence imaging analysis systems are taking the trend, but in the middle There is a big problem that most teachers are very interested in the chemiluminescence imaging analysis system, multi-color fluorescence imaging analysis system, and many of them have already done this direction, but these teachers have not been exposed to chemiluminescence imaging analysis. Systematic, multi-color fluorescence imaging analysis systems do not have sufficient knowledge of their specific functions and operations, especially in the selection of fluorescent dyes and the handling of actual animals and various samples.
4 Based on the above three points, the choice of molecular imaging imaging analysis system should follow the following principles
The product line of A molecular imaging imaging analysis system should have the long-term product line of common gel imaging analysis system chemiluminescence imaging analysis system, multi-color fluorescence imaging analysis system and multi-function living imaging analysis system, so that it can give enough teachers Choice of space. And from the ordinary gel imaging analysis system can be upgraded to the space of the chemiluminescence imaging analysis system, from the chemiluminescence imaging analysis system to the multi-color fluorescence imaging analysis system space, from multi-color fluorescence imaging analysis system to multi-function live imaging Analyze the space of the system.
The brand selected by B should have the ability to independently produce the main components of the molecular imaging imaging analysis system, especially the main parts of CCD production, filter lenses and multi-function software, so that the price of future repair parts for customers is lower. And the guarantee of quality, at the same time, the price of such a complete set of instruments can also be reduced.
The brand of C molecular imaging imaging analysis system* has the production capacity of molecular imaging imaging system and digital imaging system, or a combination of molecular imaging imaging system and digital imaging system, because the digital imaging system is now widely used in various fields. Extensive, the combination of molecular imaging imaging systems and digital imaging systems must be a new trend.
D brand imaging analysis system brand selection, especially when selecting chemiluminescence imaging analysis system, multi-color fluorescence imaging analysis system, multi-function live imaging analysis system, pay special attention to whether the brand has strong technical support, because Nowadays, chemiluminescence imaging analysis systems, multi-color fluorescence imaging analysis systems, and multi-function in vivo imaging analysis systems are not yet common. These aspects of knowledge and technology are not yet widespread, especially in the multi-function in vivo imaging analysis system.