NaCl induces salt-tolerant Populus euphratica and salt-sensitive masses to change ion flux in root cells and tissues

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The salinization of the soil is very serious, causing many agricultural and environmental problems. How to study the response of plants to salt-alkali stress and find plant varieties that can resist salt-alkali stress is one of the means to deal with soil salinization. The mechanism of salt stress on plants has been studied in the past. However, the salt stress and salt resistance of woody plants are not only difficult, but the understanding of the mechanism is stagnant.

Chen Shaoliang Laboratory of Beijing Forestry University used "non-invasive micro-testing technology (SIET)" to determine the H + of the salt-tolerant varieties of Populus euphratica and salt-sensitive varieties of poplar roots, cells and protoplasts. , Na + and Cl - flow rates, comparing the ability of Na + in the two varieties after short-term treatment and long-term treatment. Long-term stress leads to an increase in Na + outflow from root protoplasts and an increase in H + influx. Such NaCl-induced Na + / H + exchange are suppressed Amiloride and sodium alum, described the stress is discharged Populus Na + Na + / H + antiporter proactively transmembrane results. In contrast, the Na + / H + antiporter is lacking in the root tissue and cell levels of the masses after salt stress. In addition, the salt-stressed Populus euphratica retains a higher Cl - capacity than the poplar. Populus roots NaCl induced H +, Na + and Cl - in the different streams and isotonic mannitol caused by ion flow, the ion current indicates that the transition is mainly caused by the ion effect.

In this study, the H + , Na + and Cl − flows of poplar under salt stress were obtained by non-invasive micro-test technique. It was found that the salt-tolerant Populus euphratica had strong Na + and C l - The ability, as well as the Na + /H + anti-transporter living body, is one of the key factors that canopy can tolerate high salt stress. At the same time, this study established a systematic non-invasive micro-testing experimental method for plant stress research, which laid the foundation for the research in this field.

Key words: Populus euphratica, ion flow rate, Na + /H + anti-transporter, protoplast

References: Sun J, et al . Plant Physiology, 2009, 149: 1141 - 1153.

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NaCl-Induced Alternations of Cellular and Tissue Ion Fluxes in Roots of Salt-Resistant and Salt-Sensitive Poplar Species

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