Cordyceps high-yield cultivation techniques

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Cordyceps militaris is a fungus with high nourishing and medicinal value and has a promising market. Reasonable basic carbon-nitrogen ratio is a necessary condition for artificial cultivation of Cordyceps militaris. The use of rice as a culture medium provides a carbon source and a nitrogen source necessary for growth, and the addition of an antifouling agent solves the problem of contamination of the bacteria in the production of edible fungi. This is the two highlights disclosed in this article and is worthy of reader attention.

Cordyceps militaris is also known as Cordyceps militaris. It belongs to the cryptosporidium family of the ascomycetes and the ergot family. In recent years, wild resources have become scarce. However, artificial Cordyceps militaris has been well received by consumers because of its good health care functions. After many years of experiments, the authors have developed a new technology that is simple, non-polluting, and less investment, uses rice as the main raw material, and uses plastic bags to cultivate Cordyceps militaris.

I. Biological characteristics of Cordyceps militaris 1. Nutrition: First, the main carbon sources available to Cordyceps militaris are glucose, sucrose, maltose, lake starch, pectin, etc., but the use of monosaccharides or small molecule disaccharides Better. Secondly, nitrogen is an essential element for the synthesis of protein and nucleic acid substances by Cordyceps militaris. The nitrogen species, especially organic nitrogen, can be used by the North Cordyceps species, such as peptone, bean cake powder and yeast extract, etc. Inorganic nitrogen, but the use of organic nitrogen is good. Once again, in the growth of the Cordyceps mycelia and its subunits, the essential element is the mineral element. Therefore, it should be added in the production. Fourth, the appropriate amount of auxin effectively stimulates and promotes the growth of Cordyceps militaris, and greatly increases its biomass. The production of vitamins is the main measure. Finally, a reasonable substrate carbon-nitrogen ratio (CN) is a necessary condition for the artificial cultivation of Cordyceps militaris. Otherwise, it will lead to slow growth of mycelium, or serious contamination, or excessive aerial hyphae, and it is difficult for child seats to occur, even with children. Seated, the number and quality of its products have declined to varying degrees. It is advisable to adjust the CN between 3-4:1 in production.

2, temperature: Cordyceps mycelial growth temperature of 6-30C, the optimum temperature of 18-22C; primordium differentiation temperature between 10-25C, the production of timely stimulation of the formation of the primordium, can properly regulate the temperature difference in 24 The range of 5-10C within the hour; the growth temperature of the sub-seat is 10-25C, the optimum is 20-23C; the spore ejection temperature is 28-32C, and the maximum should be controlled below 34C; if non-breeding or separation and special needs, cultivation alone, Do not reach or exceed 28C for the purpose of obtaining a sub-entity.

3. Moisture: Moisture is an important foundation for life. Northern Cordyceps is no exception. As the essential solvent for the mycelium and its sub-bases during the life course, most of the water required for Cordyceps militaris comes from the culture medium. It is directly related to the growth and development of Cordyceps militaris that the matrix moisture content is appropriate. In actual production, the matrix moisture content should be adjusted to 60%-65%, and the relative air humidity during the mycelia culture period should be maintained at 60%-70%, and after forming the primordium, it should be adjusted to 80%-90%.

4. Oxygen: Cordyceps militaris is similar to other edible fungi. The process of growth and development is also a metabolic process of oxygen absorption and carbon discharge, especially after the primordial differentiation requires more oxygen, so it needs to maintain relatively fresh air to ensure oxygen. Sufficient supply. However, because Cordyceps militaris has a slow growth rate and a small metabolic amount, coupled with its relatively high ability to withstand carbon dioxide, the drop in carbon dioxide concentration in production can be met at about 0.5%.

5. Light: The early stage of growth of Cordyceps militaris is the stage of hyphae development. Light, especially strong light, has a greater effect on the inhibition and damage of mycelium. Therefore, it should be kept completely dark, even if it is near the time when the germs are completed. It will also induce premature entry into the reproductive phase, which will result in a serious reduction in output and compromise in quality. After full of bacteria, it takes about 200 lux to stimulate the color change. If the light is even and the daily time reaches 10 hours or more, the mycelium will turn color well and the color will be deep. This will lay the foundation for obtaining high-quality fruit bodies in the later period. This characteristic is consistent with the fact that wild Cordyceps does not occur in the dense shade. After the occurrence of the primordium, the illumination time can be appropriately shortened, the light intensity can be increased to 300 lux, and the light illumination can be uniform so that the fruit body can grow normally.

6, pH: Cordyceps suitable acidic environment, mycelium growth stage to adapt to the 5-7 matrix, the optimum between 5.2-6.8, artificial cultivation, the substrate pH can be adjusted to 7-7.5, after autoclaving, The pH drops naturally, and the pH of the substrate can be reduced to about 6 by adjusting the natural (produced organic acid) hyphae at the later stage.

2. Production of strains 1. Obtaining provenances with strong adaptability to seed sources and high rates of grassing are one of the key conditions for the successful cultivation of Cordyceps militaris. In addition to conditional self-separation and cultivation, most producers need access through the introduction channels. . It is recommended that the production households should have the scientific research strength, test conditions, strict, honest, and formal units introduced, “good seed mountain good seedlings” to ensure the production effect, 2 strains of transfer formula: glucose 10 g, peptone 10 g, yeast extract 2 g, asparagine 1 g, potassium dihydrogen phosphate 1.5 g, magnesium sulfate 0.6 g, auxin 5 ml, agar 15-20 g, water 1000 ml, pH 6. Routine sterilization and other operations, the use of seed sources have been prepared to inoculate, generally each seed can transfer 30 or so, the specific process will not repeat them. After cultured under 23-25C conditions, about 10 days or so, the hyphae are covered with a slant, which means that it is a primary mother or a secondary female parent; if the seed source is a F0 class that is isolated and cultured in a white line, then it is transferred after The strain is F1, that is, the first-class mother can continue to re-transform and produce F2, that is, the second-grade mother; if the provenance is purchased at the F1 level, the transferred strain is F2. That is, the secondary mother species. The secondary mother species can only be used for cultivation and production, and must not be further transferred. Otherwise, it will lead to serious consequences of “grassiness” that is sparse, sparse, or even impossible at all.

3. The production of liquid strains has many advantages such as large production volume, short production cycle, easy manual control, rapid bacterial growth after cultivation, and three-dimensional occupation of material surface by mycelium. Now it has become the preferred method for cultivation of Cordyceps militaris.

4. Selection of strains: The selection of fast-growing, high-yield and high-quality strains that are white, highly adaptable, quick-to-light, fast-growing, and stable in character are the key to successful cultivation and high efficiency. Beginners should purchase solid seed species with a large amount of bacteria, and those who have experience in cultivation can purchase beveled tube species or liquid strains.

Third, the raw material requirements 1, the choice of fresh mildew-free, pollution-free rice or corn, Northeast Cordyceps producers are now mostly used as a medium for wheat, not only the ideal yield, and Cordyceps color is better.

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